Lab Information

Edward K.L. Chan, Ph.D.

Oral Biology

Professor

Phone: 352-273-8849
E-mail: echan@ufl.edu

Address:
P.O. Box 100424
Gainesville, FL 32610-0424

Shipping Address:
Department of Oral Biology
University of Florida College of Dentistry
Health Science Center
1395 Center Dr
Rm D5-19
Gainesville, FL 32610-0424
USA

Research Training and Experience

From Research Fellow to the rank of Associate Professor, The Scripps Research Institute, 1984–2002
Ph.D. in Medical Science, University of Calgary, 1984
M.S. in Clinical Chemistry, University of Calgary, 1980
B.S. in Biochemistry, University of British Columbia, 1976

Teaching Responsibilities

Director, GMS 5905/DEN 8290: RNA interference and microRNAs: from advances in cell biology to therapeutic applications in the treatment of human diseases; Course Summary
DEN 6128: Structure and Function of Body System II: Host Defenses, lecture on Autoimmunity and Autoimmune Diseases
GMS 6161: Oral Biology II: Hypersensitivity and Autoimmune Diseases
DEN 6678: Advanced Oral Medicine: Oral Cancer and microRNAs

Active Professional Service

Vice Chair (2005-2010); Treasurer (2010-present): Autoantibody Standardization Committee in Rheumatic and Related Disorders of the International Union of Immunological Societies (IUIS)

Selected Publications

Carcamo, W.C., Satoh, M., Kasahara, H., Terada, N., Hamazaki, T., Chan, J.Y., Yao, B., Tamayo, S., Covini, G., von Mühlen, C.A., and Chan, E.K.L. (2011) Induction of cytoplasmic rods and rings structures by inhibition of the CTP and GTP synthetic pathway in mammalian cells. PLoS One 6:e29690.
Patel, R.S., Jakymiw, A., Yao, B., Pauley, B.A., Carcamo, W.C., Katz, J., Cheng, J.Q., and Chan, E.K.L. (2011) High resolution of microRNA signatures in human whole saliva. Arch. Oral Biol., 56:1506-13.
Satoh, M., Vázquez del Mercado, M., Krzyszczak, M.E., Li, Y., Ceribelli, A., Burlingame, R.W., Webb, T.T., Sobel, E.S., Reeves, W.H., and Chan, E.K.L. (2011) Coexistence of anti-RNA polymerase III and anti-U1RNP antibodies in patients with SLE - Two cases without features of scleroderma. Lupus, 21:68-74.
Nahid, M.A., Satoh, M., and Chan, E.K.L. (2011) Mechanistic role of miR-146a in endotoxin-induced differential cross-regulation of TLR signaling. J Immunol. 186:1723-34.
Nahid, M.A., Rivera, M., Lucas, A., Chan, E.K.L., and Kesavalu, L. (2011) Polymicrobial infection with periodontal pathogens specifically enhances miR-146a in ApoE-/- mice during experimental periodontal disease. Infection Immun. 79:1597-605.
Satoh, M., Bjerkås, I., Haugarvoll, E., Chan, E.K.L., Szabo, N.J., Jirillo, E., Poppe, T.T., Sveier, H., Tørud, B., and Koppang, E.O. (2011) Polyclonal hypergammaglobulinemia and autoantibody production induced by vaccination in farmed Atlantic salmon. Fish Shellfish Immunol. 30:1080-6. PMID: 21316456.
Yao, B., Li, S., Lian, S.L., Fritzler, M.J., and Chan, E.K.L. (2011) Mapping of Ago2-GW182 functional interactions. Methods Mol. Biol. 725:45-62. PMID: 21528446.
Satoh, M., Krzyszczak, M.E., Li, Y., Ceribelli, A., Ross, S.J., Chan, E.K.L., Segal, M.S., Bubb, M.R., Sobel, E.S., and Reeves, W.H. (2011) Frequent coexistence of anti-topoisomerase I and anti-U1RNP autoantibodies in African American patients associated with mild skin involvement: a retrospective clinical study. Arthritis Res. Ther. 13:R73. PMID: 21569292.
Satoh, M., Chan, J.Y.F., Ross, S., Ceribelli, A., Cavazzana, I., Franceschini, F., Li, Y., Reeves, W.H., Sobel, E.S., and Chan, E.K.L. (2011) Autoantibodies to survival of motor neuron complex in patients with polymyositis: Immunoprecipitation of D, E, F, and G proteins without other components of small nuclear ribonucleoproteins. Arthritis Rheum., 63:1972-8. PMC3123691 PMID: 21425128.
Pauley, K.M., Stewart, C.M., Gauna A.E., Dupre, L.C., Kuklani, R., Chan, A.L., Pauley, B.A., Reeves, W.H., Chan, E.K.L., and Cha, S. (2011) Altered miR-146a expression in Sjögren's Syndrome and its functional role in innate immunity. European J. Immunol., 41:2029-39.
Ceribelli, A., Krzyszczak, M.E., Li, Y., Ross, S.J., Chan, J.Y.F., Chan, E.K.L., Burlingame, R., Webb, T.T., Bubb, M.R., Sobel, E.S., Reeves, W.H., and Satoh, M. (2011) Atypical clinical presentation of a subset of patients with anti-RNA polymerase III - non-scleroderma cases associated with dominant RNA polymerase I reactivity and nucleolar staining. Arthritis Res. Ther. 13:R119.
Pauley, K.M., Gauna A.E., Grichtchenko, I.I., Chan, E.K.L., and Cha, S. (2011) A secretagogue-small interfering RNA conjugate confers resistance to cytotoxicity in a cell model of Sjögren's Syndrome. Arthritis Rheum. 63:3116-25.
Krzyszczak, M.E., Li, Y., Ross, S.J., Ceribelli, A., Chan, E.K.L., Bubb, M.R., Sobel, E.S., Reeves, W.H., and Satoh, M. (2011) Gender and ethnicity differences in the prevalence of scleroderma-related autoantibodies. Clin. Rheumatol. 30:1333-9.
Yao, B., Li. S., Jung, H.M., Lian, S.L., Abadal, G.X., Han, F., Fritzler, M.J., and Chan, E.K.L. (2011) Divergent GW182 functional domains in the regulation of translational silencing. Nucleic Acids Res. 39:2534-47. PMC3074120.
Ceribelli, A., Tincani, A., Cavazzana, I., Franceschini, F., Cattaneo, R., Pauley, B.A., Chan, J.Y.F., Chan, E.K.L., and Satoh, M. (2011) Anti-argonaute2 (Ago2/Su) and -Ro antibodies identified by immunoprecipitation in primary anti-phospholipid syndrome (PAPS). Autoimmunity 44:90-7.
Vázquez-Del Mercado, M., Sánchez Orozco, L.V., Pauley, B.A., Chan, J.Y.F., Chan, E.K.L., Panduro, A., Maldonado González, M., Jimenez-Luevanos, M.A., Martin Márquez, B.T., Palafox-Sanchez, C.A., Dávalos-Rodríguez, I.P., Salazar-Páramo, M., González Lopez, L., Gámez Nava, J.I., and Satoh, M. (2010) Autoantibodies to a miRNA-binding protein Argonaute 2 (Su antigen) in patients with hepatitis C virus infection. Clin. Exp. Rheumatol. 28:842-8.
Katz, J., Jakymiw, A., Ducksworth, M.K., Stewart, C.M., Bhattacharyya, I., Cha, S., and Chan, E.K.L. (2010) CIP2A expression and localization in oral carcinoma and dysplasia. Cancer Biology & Therapy 10:694-699.
Ceribelli, A., Cavazzana, I., Franceschini, F., Airò, P., Tincani, A., Cattaneo, R., Pauley, B.A., Chan, E.K.L., and Satoh, M. (2010) Anti-Th/To are common anti-nucleolar autoantibodies in Italian scleroderma patients. J. Rheumatol. 37:2071-5.
Jakymiw, A., Patel, R., Deming, N., Bhattacharyya, I., Shah, P., Lamont, R.J., Stewart, C., Cohen, D., and Chan, E.K.L. (2010) Overexpression of dicer as a result of reduced let-7 microRNA levels contributes to increased cell proliferation of oral cancer cells. Genes, Chromosomes and Cancer 49:540-59. PMC2859695.
Pauley, K.M., Satoh, M., Pauley, B.A., Dominguez-Gutierrez, P.R., Wallet, S.M., Holliday, L.S., Cha, S., Reeves, W.H., and Chan, E.K.L. (2010) Formation of GW/P bodies as marker for microRNA-mediated regulation of innate immune signaling in THP-1 cells. Immunol. Cell Biol. 88:205-12. PMC2824770.
Vázquez-Del Mercado, M., Palafox-Sánchez, C.A., Muñoz-Valle, J.F., Orozco-Barocio, G., Oregon Romero, E., Navarro Hernández, R.E., Salazar-Páramo, M., Chan, J.Y.F., Chan, E.K.L., and Satoh, M. (2010) High prevalence of autoantibodies to RNA helicase A in Mexican patients with systemic lupus erythematosus. Arthritis Res. Ther. 12:R6. PMC2875632.
Pereira, D.B., Sannes, T., Dodd, S.M., Jensen, S.E., Morgan, L.S., and Chan, E.K.L. (2010) Life stress, negative mood states, and antibodies to heat shock protein 70 in endometrial cancer. Brain, Behavior, and Immunity 24:210-4. PMC2818111.
Ostrov, D.A., Magis, A.T., Wronski, T.J., Chan, E.K.L., Toro, E.J., Donatelli, R.E., Sajek, K., Haroun, I.N., Nagib, M.I. Piedrahita, A., Harris, A., and Holliday, L.S. (2009) Identification of enoxacin as an inhibitor of osteoclast formation and bone resorption by structure-based virtual screening. J. Med. Chem. 52:5144-5151. PMC2889180.
Kakumanu, P., Sobel, E.S., Narain, S., Li, Y., Akaogi, J., Yamasaki, Y., Segal, M.S., Hahn, P.C., Chan, E.K.L., Reeves, W.H., and Satoh, M. (2009) Citrulline-dependence of anti-CCP antibodies in SLE as a marker of deforming/erosive arthritis. J. Rheumatol. 36:2682-90. PMC2803319.
Nahid, M.A., Pauley, K.M., Satoh, M., and Chan, E.K.L. (2009) MicroRNA-146a is critical for endotoxin-induced tolerance. Implication on innate immunity. J. Biol. Chem. 284:34590-34599. PMC2787321.
Brucato, A., Grava, C., Bortolati, M., Ikeda, K., Milanesi, O., Cimaz, R., Ramoni, V., Vignati, G., Martinelli, S., Sadou, Y., Borghi, A., Tincani, A., Chan, E.K.L., and Ruffatti, A. (2009) Congenital Heart Block not associated with anti-Ro/La antibodies: comparison with anti-Ro/La-positive cases. J. Rheumatol. 36:1744-8. PMC2798588.
Lian, S.L., Li. S., Abadal, G.X., Pauley, B.A., Fritzler, M.J., and Chan, E.K.L. (2009) The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing. RNA 15:804-13. PMC2673069.
Moser, J.J., Chan, E.K.L., and Fritzler, M.J. (2009) Optimization of immunoprecipitation-western blot analysis in detecting GW182-associated components of GW/P Bodies. Nat Protoc. 4:674-85. PMC2797048.
Llanos, C., Chan, E.K.L., Li, S., Abadal, G.X., Izmirly, P., Byrne, C., Clancy, R.M., and Buyon, J.P. (2009) Antibody Reactivity to α-Enolase in Mothers of Children with Congenital Heart Block. J. Rheumatol. 36:565-9.
Palafox-Sánchez, C.A., Satoh, M., Chan, E.K.L., Carcamo, W.C., Muñoz-Valle, J.F., Orozco-Barocio, G., Oregón-Romero, E., Navarro-Hernández, R.E., Salazar-Páramo, M., Castañeda, A.C., and Vázquez-Del Mercado, M. (2009) Reduced IgG anti-snRNPs autoantibody production in SLE patients with positive IgM anti-cytomegalovirus antibodies. Arthritis Res. Ther. 11:R27. PMC2688261.
Nozawa, K., Ikeda, K., Satoh, M., Reeves, W.H., Stewart, C., Li, Y.C., Yen, T.J., Rios, R.M., Takamori, K., Ogawa, H., Sekigawa, I., Takasaki, Y., and Chan, E.K.L. (2009) Autoantibody to NA14 is an independent marker primarily for Sjögren's Syndrome. Frontiers Biosci. 14:3733-3739. PMC2864436.
Bulosan, M., Pauley, K.M., Yo, K., Chan, E.K.L., Katz, J., Peck, A.B., and Cha, S. (2009) Inflammatory caspases are critical for enhanced cell death in the target tissue of Sjögren's Syndrome prior to disease onset. Immunol. Cell Biol. 87:81-90.
Nozawa, K., Fritzler, M.J., Takasaki, Y., Wood, M.R., and Chan, E.K.L. (2009) Co-clustering of Golgi complex and other cytoplasmic organelles to crescentic region of half-moon nuclei during apoptosis. Cell Biol. Int. 33:148-157. PMC2667205
Li, S., Lian, S.L., Moser, J.J., Fritzler, M.L., Fritzler, M.J., Satoh, M., and Chan, E.K.L. (2008) Identification of GW182 and its novel isoform TNGW1 as translational repressors in Ago2 mediated silencing. J. Cell Sci. 121:4134-44.
Pauley, K.M., Satoh, M., Chan, A.L., Bubb, M.R., Reeves, W.H., and Chan, E.K.L. (2008) Upregulated miR-146a expression in peripheral blood mononuclear cells from rheumatoid arthritis patients. Arth. Res. Ther. 10:R101.
Lian, S., Fritzler, M.J., Katz, J., Hamazaki, T., Terada, N., Satoh, M., and Chan, E.K.L. (2007) Small interfering RNA-mediated silencing induces target-dependent assembly of GW/P bodies. Mol. Biol. Cell 18:3375-87. Expert Reviews written by Herrera-Esparza et al, 2008
Zhang, J.Y., Megliorino, R., Peng, X.X., Tan, E.M., Chen, Y., and Chan, E.K.L. (2007) Antibody detection using tumor-associated antigen mini-array in immunodiagnosing human hepatocellular carcinoma. J. Hepatol. 46:107-114.
Ikeda, K., Satoh, M., Pauley, K.M., Fritzler, M.J., Reeves, W.H., and Chan, E.K.L. (2006) Detection of the argonaute protein Ago2 and microRNAs in the RNA induced silencing complex (RISC) using a monoclonal antibody. J. Immunol. Methods 317:38-44.
Pauley, K.M., Eystathioy, T., Jakymiw, A., Hamel J.C., Fritzler, M.J., and Chan, E.K.L. (2006) Formation of GW bodies is a consequence of miRNA genesis. EMBO reports 7:904-910.
Jakymiw, A., Ikeda, K., Fritzler, M.J., Reeves, W.H., Satoh, M., and Chan, E.K.L. (2006) Autoimmune targeting of key components of RNA interference. Arthritis Res. Ther., 8:R87.
Lian, S., Jakymiw, A., Eystathioy, T., Hamel, J.C., Fritzler, M.J., and Chan, E.K.L. (2006) GW bodies, microRNAs and the cell cycle. Cell Cycle 5:242-5.
Jakymiw, A., Lian, S., Eystathioy, T., Li, S., Satoh, M., Hamel J.C., Fritzler, M.J., and Chan, E.K.L. (2005) Disruption of GW bodies impairs mammalian RNA Interference. Nature Cell Biol. 7:1267-74.

Selected Chapters, Reviews & Editorials

Chan, E.K.L., Ceribelli, A., and Nahid, M.A. (2011) The role of miRNAs in the regulation of immunological functions and the prevention of autoimmunity. In: K. Conrad, E.K.L. Chan, M.J. Fritzler, R.L. Humbel, P.L. Meroni, Y. Shoenfeld (eds), From Prediction to Prevention of Autoimmune Diseases. Autoantigens, Autoantibodies and Autoimmunity series. Volume 7. Proceedings 10th Dresden Symposium on Autoantibodies. Pabst Science Publishers, Lengerich, Germany, pp253-266.
Ceribelli, A. Nahid, M.A., Satoh, M., and Chan, E.K.L. (2011) MicroRNAs in rheumatoid arthritis. FEBS Letters 585, pp. 3667-3674.
Ceribelli, A., Yao, B., Dominguez-Gutierrez, P.R., and Chan, E.K.L. (2011) Editorial: Lupus T cells switched on by DNA hypomethylation via microRNAs? Arthritis Rheum. 63:1177-81. NIHMS257696 PMID: 21538310
Ceribelli, A., Yao, B., Dominguez-Gutierrez, P.R., Nahid, M.A., Satoh, M., and Chan, E.K.L. (2011) MicroRNAs in systemic rheumatic diseases. Arthritis Res. Ther. 13:229.
Nahid, M.A., Satoh, M., and Chan, E.K.L. (2011) MicroRNA in TLR signaling and endotoxin tolerance. Cell. Mol. Immunol. 8:388-403.
Chan, E.K.L. (2010) Autoantibodies to multiple components of the microRNA pathway and aberrant microRNA expression in autoimmune diseases. In: Jack D. Keene (ed), Autoantibodies and Autoimmunity, MBL, Tokyo, Japan, pp45-59.
Satoh, M., Fritzler, M.J., and Chan, E.K.L. (2010) Anti-histone and anti-spliceosomal antibodies. In Systemic Lupus Erythematosus, 5th ed. edited by Robert G. Lahita. Academic Press, San Diego, Chapter 16, pp. 275- 292.
Katz, J. and Chan, E.K.L. (2009) Commentary: Cell cycle proteins to monitor cancer of salivary glands.Cancer Biol. Ther. 8:159-160.
Satoh, M., Vázquez-Del Mercado, M., and Chan, E.K.L. (2009) Clinical interpretation of antinuclear antibody tests in systemic rheumatic diseases. Mod. Rheum. 19:219-28. PMC2876095
Chan, E.K.L., Satoh, M., and Pauley, K.M. (2009) Editorial: Contrast in aberrant microRNA expression in SLE and RA: Is miR-146 all we need? Arthritis Rheum. 60:912-915. PMC2792591
Pauley, K.M. Cha, S., and Chan, E.K.L. (2009) MicroRNA in autoimmunity and autoimmune diseases. J. Autoimmunity 32:189-194. PMC2717629
Pauley, K.M., and Chan, E.K.L. (2008) MicroRNAs and their emerging roles in immunology. In: Noel R. Rose (ed), The Year in Immunology, Ann. N.Y. Acad. Sci. 1143:226-39.
Jakymiw, A., Pauley, K.M., Li, S., Lian, S., Ikeda, K., Eystathioy, T., Satoh, M., Fritzler, M.J., and Chan, E.K.L. (2007) The role of GW/P-bodies in RNA processing and silencing. J. Cell Sci., 120:1317-1323.