Faculty Pages - UF College of Dentistry

Jeffrey D. Hillman

Oral Biology

Professor
(352) 846-0792
jhillman@dental.ufl.edu

Ph.D., Harvard University,
    Microbiology and Molecular Genetics, 1976

D.M.D., Harvard School of Dental Medicine,
    Dentistry, 1973

University of Chicago, Biology

Main Interests:

Molecular genetic approach to the following research topics:

Effector strain construction for the prevention of bacterial infections using replacement therapy.
Purification and characterization of novel lantibiotics from Streptococci.
Construction andtesting of several novel in vivo expression systems to identify new bacterial virulence factors.
Microbial ecology of the oral cavity.

Industrial Relevance
Three separate technologies have been patented:

Replacement therapy for the prevention of dental caries using a genetically engineered strain of Streptococcus mutans for use as an effector strain.
A novel anitibiotic called mutacin 1140 that is stable and has a broad spectrum of activity.
In vivo induced antigen technology (IVIAT) for the identification of genes of pathogenic microorganisms specifically expressed during an actual infection in human.

Publications:

Salim, K.Y., Cvitkovitch, D.G., Chang, P., Bast, D.J., Handfield, M., Hillman, J.D., & de Azavedo, J.C. (2005). Identification of group A Streptococcus antigenic determinants upregulated in vivo. Infect Immun, 73(9), 6026-38.

Song, Y.H., Kozarov, E.V., Walters, S.M., Cao, S.L., Handfield, M., Hillman, J.D., & Progulske-Fox, A. (2002). Genes of periodontopathogens expressed during human disease. Ann Periodontol, 7(1), 38-42.

Yuan, L., Hillman, J.D., & Progulske-Fox, A. (2005). Microarray analysis of quorum-sensing-regulated genes in Porphyromonas gingivalis. Infect Immun, 73(7), 4146-54.

Handfield, M., Progulske-Fox, A., & Hillman, J.D. (2005). In vivo induced genes in human diseases. Periodontol 2000, 38, 123-34.

John, M., Kudva, I.T., Griffin , R.W., Dodson, A.W., McManus, B., Krastins, B., Sarracino, D., Progulske-Fox, A., Hillman, J.D., Handfield, M., Tarr, P.I., & Calderwood, S.B. (2005). Use of in vivo-induced antigen technology for identification of Escherichia coli O157, H7 proteins expressed during human infection. Infect Immun, 73(5), 2665-79.

Rollins, S.M., Peppercorn, A., Hang, L., Hillman, J.D., Calderwood, S.B., Handfield, M., & Ryan, E.T. (2005). In vivo induced antigen technology (IVIAT). Cell Microbiol, 7(1), 1-9.

Cao, S.L., Progulske-Fox, A., Hillman, J.D., & Handfield, M. (2004). In vivo induced antigenic determinants of Actinobacillus actinomycetemcomitans. FEMS Microbiol Lett, 237(1), 97-103.

Kim, Y.R., Lee, S.E., Kim, C.M., Kim, S.Y., Shin, E.K., Shin, D.H., Chung, S.S., Choy, H.E., Progulske-Fox, A., Hillman, J.D., Handfield, M., & Rhee, J.H. (2003). Characterization and pathogenic significance of Vibrio vulnificus antigens preferentially expressed in septicemic patients. Infect Immun, 71(10), 5461-71.

Smith, L., Zachariah, C., Thirumoorthy, R., Rocca, J., Novak, J., Hillman, J.D., & Edison, A.S. (2003). Structure and dynamics of the lantibiotic mutacin 1140. Biochemistry, 42(35), 10372-84.

Hang, L., John, M., Asaduzzaman, M., Bridges, E.A., Vanderspurt, C., Kirn, T.J., Taylor , R.K., Hillman, J.D., Progulske-Fox, A., Handfield, M., Ryan, E.T., & Calderwood, S.B. (2003). Use of in vivo-induced antigen technology (IVIAT) to identify genes uniquely expressed during human infection with Vibrio cholerae. Proc Natl Acad Sci U S A, 100(14), 8508-13.

Cheng, S., Clancy, C.J., Checkley, M.A., Handfield, M., Hillman, J.D., Progulske-Fox, A., Lewin, A.S., Fidel, P.L., & Nguyen, M.H. (2003). Identification of Candida albicans genes induced during thrush offers insight into pathogenesis. Mol Microbiol, 48(5), 1275-88.

Handfield, M., Seifert, T., & Hillman, J.D. (2003). In vivo expression of bacterial genes during human infections. Methods Mol Med, 71, 225-42.

Hillman, J.D. (2002). Genetically modified Streptococcus mutans for the prevention of dental caries. Antonie Van Leeuwenhoek, 82(1-4), 361-6.

Wu, Y., Lee, S.W., Hillman, J.D., & Progulske-Fox, A. (2002). Identification and testing of Porphyromonas gingivalis virulence genes with a pPGIVET system. Infect Immun, 70(2), 928-37.